|Manga Debut||Act 110|
|Anime Debut||Episode 48|
Tsubaki is the daughter of the late village chief and one of four orphans taken in by Yūkyūzan Anji. Being the eldest of the 4 children, she is always protective of them and has deep affection for Anji, wanting him to stay with her. She was burned alive along with the other children under orders of the village chief, due to the Meiji Government's ban of Buddhism and because the parents of the 4 orphans sided with the Shogunate during the Boshin wars. Anji's spirit shatters after the murder of the children and he later kills the village chief in revenge.